Taking place in the middle of London Fashion Week at the city's iconic St. Paul's Cathedral, near where McQueen grew up, at times the service resembled a fashion show as several of the late designer's friends arrived in their finest clothes.
Naomi Campbell turned heads in black feathers and leather, while fellow supermodel Kate Moss opted for a black leather minidress and tuxedo jacket, which still allowed for plenty of cleavage.
Inside the cathedral Icelandic performer Björk, dressed in white and wearing angel wings, sang "Gloomy Sunday," a composition associated with suicide.
Wintour, who spoke during the service, remembered McQueen as "a complex and gifted young man."
"His final collection was a battle between dark and light. His was an 18-year career of harnessing his dreams and demons," she said.
"He showed us everything was possible; dreams could become reality. But he has left us with an even more exceptional legacy, a talent that soared like the birds of his childhood above us all."
McQueen, 40, stunned the fashion world by taking his own life in February. The designer, who reportedly took a cocktail of drugs before hanging himself, had been depressed by the recent death of his mother.
He was remembered in March on what would have been his 41st birthday by a number of prominent New York-based designers including Diane von Furstenberg, Michael Kors, Zac Posen and Calvin Klein's Francisco Costa.
The Alexander McQueen label lives on under the creative direction of Sarah Burton.