Madonna stunned fans in Istanbul, Turkey on Saturday night when she bared her right breast during her latest stop on the MDNA tour.

The pop superstar was in the middle of performing her hit Human Nature when she purposely flashed her nipple in what clearly wasn't a Janet Jackson-esque wardrobe malfunction.

Fans roared their approval as the defiant 53-year-old singer made it clear she didn't care who she offended, turning her back on the audience to show off a temporary 'NO FEAR' tattoo.

madonna nipple

Oops: Madonna shows off her support act

madonna

A youthful looking Madonna during her tour

The cheeky split-second nipple flash came as Madonna performed a striptease onstage.

It's not the first time she has sparked controversy since her MDNA tour got underway in Tel Aviv, Israel - she upset French National Front leader Marine Le Pen during one show last week when she projected an image of a Nazi swastika on the politician's forehead onto large screens around the stage.

Le Pen has threatened to sue Madonna if the incident is repeated when the pop star hits the stage in France in July.

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  • Madonna

    U.S pop icon Madonna at the Ramat Gan stadium near Tel Aviv, Israel, Thursday, May 31, 2012. Madonna launch her hotly-anticipated MDNA world tour with a show in Tel Aviv, kicking off a roadtrip of more than 30 countries in what will be her longest-ever stint on the road. (AP Photo/Ariel Schalit)

  • TEL AVIV, ISRAEL - MAY 31: Madonna performs on stage during her "MDNA" tour at Ramat Gan Stadium on May 31, 2012 in Tel Aviv, Israel. (Photo by Kevin Mazur/WireImage)

  • US pop icon Madonna (C) performs on stag

    US pop icon Madonna (C) performs on stage during her first MDNA world tour concert in the Ramat Gan Stadium, near Tel Aviv, on May 31, 2012. Madonna's Tel Aviv concert sold 32,000 tickets and it is the first in her world tour spanning 30 countries and 86 concert dates to promote her new MDNA album. AFP PHOTO / JACK GUEZ (Photo credit should read JACK GUEZ/AFP/GettyImages)

  • TEL AVIV, ISRAEL - MAY 31: Madonna performs on stage during her "MDNA" tour at Ramat Gan Stadium on May 31, 2012 in Tel Aviv, Israel. (Photo by Kevin Mazur/WireImage)

  • Madonna

    U.S pop icon Madonna performs at the Ramat Gan stadium near Tel Aviv, Israel, Thursday, May 31, 2012. Pop music star Madonna is kicking off her new world tour in front of tens of thousands of ecstatic fans in Israel. (AP Photo/Ariel Schalit)

  • US pop icon Madonna performs on stage du

    US pop icon Madonna performs on stage during her first MDNA world tour concert in the Ramat Gan Stadium, near Tel Aviv, on May 31, 2012. Madonna's Tel Aviv concert sold 32,000 tickets and it is the first in her world tour spanning 30 countries and 86 concert dates to promote her new MDNA album. AFP PHOTO / JACK GUEZ (Photo credit should read JACK GUEZ/AFP/GettyImages)

  • Madonna

    U.S pop icon Madonna performs at the Ramat Gan stadium near Tel Aviv, Israel,Thursday, May 31, 2012. Pop music star Madonna is kicking off her new world tour in front of tens of thousands of ecstatic fans in Israel. (AP Photo/Ariel Schalit)

  • US pop icon Madonna (C) performs on stag

    US pop icon Madonna (C) performs on stage during her first MDNA world tour concert in the Ramat Gan Stadium, near Tel Aviv, on May 31, 2012. Madonna's Tel Aviv concert sold 32,000 tickets and it is the first in her world tour spanning 30 countries and 86 concert dates to promote her new MDNA album. AFP PHOTO / JACK GUEZ (Photo credit should read JACK GUEZ/AFP/GettyImages)

  • US pop icon Madonna (C) performs on stag

    US pop icon Madonna (C) performs on stage during her first MDNA world tour concert in the Ramat Gan Stadium, near Tel Aviv, on May 31, 2012. Madonna's Tel Aviv concert sold 32,000 tickets and it is the first in her world tour spanning 30 countries and 86 concert dates to promote her new MDNA album. AFP PHOTO / JACK GUEZ (Photo credit should read JACK GUEZ/AFP/GettyImages)

  • US pop icon Madonna performs on stage du

    US pop icon Madonna performs on stage during her first MDNA world tour concert in the Ramat Gan Stadium, near Tel Aviv, on May 31, 2012. Madonna's Tel Aviv concert sold 32,000 tickets and it is the first in her world tour spanning 30 countries and 86 concert dates to promote her new MDNA album. AFP PHOTO / JACK GUEZ (Photo credit should read JACK GUEZ/AFP/GettyImages)

  • Madonna "MDNA" World Tour Opener

    TEL AVIV, ISRAEL - MAY 31: Madonna performs during the opening concert of her 'MDNA' World Tour at Ramat Gan stadium on May 31, 2012 in Tel Aviv, Israel. (Photo by Ilia Yefimovich/Getty Images)

  • Madonna "MDNA" World Tour Opener

    TEL AVIV, ISRAEL - MAY 31: Madonna performs during the opening concert of her 'MDNA' World Tour at Ramat Gan stadium on May 31, 2012 in Tel Aviv, Israel. (Photo by Ilia Yefimovich/Getty Images)

  • Madonna

  • Madonna

    U.S pop icon Madonna performs at the Ramat Gan stadium near Tel Aviv, Israel,Thursday, May 31, 2012. Pop music star Madonna is kicking off her new world tour in front of tens of thousands of ecstatic fans in Israel. (AP Photo/Ariel Schalit)

  • Madonna

    U.S pop icon Madonna at the Ramat Gan stadium nearTel Aviv, Israel, Thursday, May 31, 2012. Madonna launch her hotly-anticipated MDNA world tour with a show in Tel Aviv, kicking off a road trip of more than 30 countries in what will be her longest-ever stint on the road. Pop music star Madonna is kicking off her new world tour in front of tens of thousands of ecstatic fans in Israel. (AP Photo/Ariel Schalit)

  • Madonna

    U.S pop icon Madonna at the Ramat Gan stadium near Tel Aviv, Israel, Thursday, May 31, 2012. Madonna launch her hotly-anticipated MDNA world tour with a show in Tel Aviv, kicking off a roadtrip of more than 30 countries in what will be her longest-ever stint on the road. (AP Photo/Ariel Schalit)

  • Israelis dance during a party in a club of Tel Aviv to celebrate the arrival of Madonna in Israel, early Saturday, May 26, 2012. The Madonna 2012 World Tour will start on May 29 in Tel Aviv. (AP Photo/Dan Balilty)

  • US pop icon Madonna performs on stage du

    US pop icon Madonna performs on stage during her first MDNA world tour concert in the Ramat Gan Stadium, near Tel Aviv, on May 31, 2012. Madonna's Tel Aviv concert sold 32,000 tickets and it is the first in her world tour spanning 30 countries and 86 concert dates to promote her new MDNA album. AFP PHOTO / JACK GUEZ (Photo credit should read JACK GUEZ/AFP/GettyImages)

  • US pop icon Madonna performs on stage du

    US pop icon Madonna performs on stage during her first MDNA world tour concert in the Ramat Gan Stadium, near Tel Aviv, on May 31, 2012. Madonna's Tel Aviv concert sold 32,000 tickets and it is the first in her world tour spanning 30 countries and 86 concert dates to promote her new MDNA album. AFP PHOTO / JACK GUEZ (Photo credit should read JACK GUEZ/AFP/GettyImages)

  • Madonna "MDNA" World Tour Opener

    TEL AVIV, ISRAEL - MAY 31: Madonna performs during the opening concert of her 'MDNA' World Tour at Ramat Gan stadium on May 31, 2012 in Tel Aviv, Israel. (Photo by Ilia Yefimovich/Getty Images)

  • TEL AVIV, ISRAEL - MAY 31: Rocco Ritchie and Madonna perform on stage during her 'MDNA' tour at Ramat Gan Stadium on May 31, 2012 in Tel Aviv, Israel. (Photo by Kevin Mazur/WireImage)

  • TEL AVIV, ISRAEL - MAY 31: Rocco Ritchie and Madonna perform on stage during her 'MDNA' tour at Ramat Gan Stadium on May 31, 2012 in Tel Aviv, Israel. (Photo by Kevin Mazur/WireImage)

  • TEL AVIV, ISRAEL - MAY 31: Rocco Ritchie and Madonna perform on stage during her 'MDNA' tour at Ramat Gan Stadium on May 31, 2012 in Tel Aviv, Israel. (Photo by Kevin Mazur/WireImage)