Two teenage girls have died following a collision with a deer on the northbound carriageway of the A10 between Ware and the A120 Puckeridge exit in Hertfordshire.
The Citroen C1 car in which the girls, who were 17 and 18, were travelling hit a deer and then a Volvo V40 collided with the Citroen, which caught fire.
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The incident occurred at around 7.45am on Tuesday.
The girls died at the scene while the driver of the Volvo, who is believed to be in his 50s, was taken to hospital for treatment for what are thought to be leg injuries.
The road was closed and remained shut into the early afternoon.
Police said anyone who witnessed the accident or had any information should contact Sergeant Richard Cruse via the Herts police non-emergency number 101.
Motoring, wildlife and safety groups repeatedly warn road users of the dangers of deer on highways. The toll of deer involved annually in vehicle collisions in the UK is estimated to be as high as 74,000.
Road accidents involving deer also result in more than 450 human injuries a year as well as several deaths.
Six main species of deer live wild in Britain with an estimated combined population of more than 1.5 million.