Emily Eavis has confirmed that a metal sculpture will adorn the Pyramid Stage, inspired by the iconic album artwork for Bowie's 'Aladdin Sane'.
The Park Stage will also play host to a performance of Philip Glass's 'Symphony No 4', which is based on the late singer's 'Heroes' album.
She also claimed that plans are afoot to hold a late-night Prince party.
"And we're doing Philip Glass's Heroes Symphony on the Park Stage on Saturday night, which is going to be a really intimate tribute to Bowie. It's being conducted by Charles Hazlewood with members of the Paraorchestra. And we're going to do an incredible light show which will go on for the whole show and beyond, into the night.
Bowie previously headlined the festival, playing the Pyramid Stage in 1971 and 2000.
Prince, however, never played Glastonbury, but Emily admitted that she came close to signing him on a number of occasions.
Speaking of his death, she said: "It's gutting to be honest. We were so shocked and sad. It's been so close to happening for the last three years. A couple of times it's been confirmed and then not happened.
"It's really disappointing that he hasn't played here but it's also, obviously, a huge loss to music because he was an incredible force. Just phenomenal. There was no-one else like him on the planet."
David Bowie died in January, aged 69, following a private battle with cancer.
Prince was found dead at his Paisley Park home in Minnesota last month, at the age of 57.
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