The rockers delighted the crowd with an electrifying set at Worthy Farm in Pilton, Somerset.
Frontman Matt Bellamy and his fellow bandmates worked their way through a 21 song setlist, including some of their biggest hits like 'Supermassive Black Hole', 'Time Is Running Out' and 'Plug In Baby'.
Revellers still have plenty left to enjoy as this year's festival continues over the next two days.
Watch Muse perform 'Mercy' at Glastonbury in the video below...
Saturday night will see Adele play her highly-anticipated headline set, which will mark the first time she has ever performed at Glastonbury.
Sunday night will also see seasoned headliners Coldplay introduce their third Pyramid Stage set, as they draw the 2016 event to a close.
Across the many other stages at the event, the likes of The 1975, Tom Odell, Years And Years and Earth, Wind And Fire will also perform.
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