The Olympic Flame has been taken across the famous Swilcan Bridge on the Old Course in the home of golf, St Andrews.
It was also used to recreate a scene from the film Chariots Of Fire at the Fife town's West Sands beach.
The torch will travel 145 miles on this leg of its symbolic journey around the UK, reaching Edinburgh Castle later today.
A total of 115 torchbearers will carry the flame past some of the country's most recognisable landmarks, including the Forth Bridge, Stirling Castle, the Wallace Monument and the Falkirk Wheel.
The Swilcan Bridge dates back some 700 years and was originally built for shepherds herding their sheep over the Swilcan Burn that now winds through the first and 18th fairways.
The small stone bridge is now one of the most famous spots in the golfing world.
At the Old Course, the torch was carried by Louise Martin, 65, from Dunblane.
Mrs Martin is chair of Sport Scotland. She said: "Now that it's over, it's quite surreal. I can't believe I've actually done it. It's churning inside, just the pleasure and what it meant to me.
"Words can't explain how I feel to have just done what I've done with the Olympic Flame in my hand."
Keen athlete Joseph Forrester, 13, ran with the flame along the West Sands.
He was followed by 20 pupils from Madras Primary School.
Joseph is a member of Fife Athletics Club and dreams of running for Team GB in long-distance events.
He said: "I was kind of nervous before it because there's loads of people here but I'm all right now.
"I'd love to compete in the Olympics one day."
The flame formally departed from the town's ancient university, where the Duke and Duchess of Cambridge studied.
Today's procession marks the 26th day of the torch's marathon relay over the length and breadth of the UK.
On the way south, the torchbearers will pass through Milnathort, Kinross, Crook of Devon, Alloa, Bridge of Allan, Dunblane, Cumbernauld, Larbert, Camelon, Skinflats, Cairneyhill, Crossford, Dunfermline and Broxburn.
Hannah Clarke, 18, from Kinross, will carry the flame in Milnathort. She was diagnosed with an inoperable tumour and has since raised nearly £10,000 for cancer charities.
Lee Hesp, 46, who supported his daughter after she was diagnosed with an invasive form of cancer in 2006, will carry the flame in Crook of Devon.
The torch will also visit Hopetoun House and the town of Broxburn, West Lothian, before veering towards Edinburgh.
It will travel along Slateford Road and Colinton Road, through Tollcross, towards Chambers Street, The Mound and the Royal Mile in the heart of the Old Town.
One of the torch carriers will be 19-year-old Jodi Mcginty, of Edinburgh, who is said to have made an "excellent contribution" to developing sport in her community.
The evening celebration at Edinburgh Castle will feature a variety of entertainment.
Singer Emeli Sande will headline the evening's events, which will also involve acrobatic performances, dance displays and community acts.
Towards the end of the two-hour show, the last torchbearer of the day, Lesley Forrest, will light a cauldron on stage.
Ms Forrest, 54, received a kidney transplant in 1996 and a year later started competing in the British Transplant Games as part of the Scottish team. She went on to become a multiple medallist in both the British and World Transplant Games.