Radamel Falcao has been left out of Colombia's World Cup squad by coach José Pékerman.
Falcao, who suffered a serious knee injury when playing for Monaco in January, was left off the 23-man list after Pékerman decided he would not be ready.
Former Atlético Madrid frontman Falcao, who was a big-money signing by Monaco last summer, looked to have no hope of making his country's squad for the tournament in Brazil when he sustained the injury, however his recovery then progressed swiftly.
Pekerman said last week he would "wait for Falcao until the last day, the last minute" before deciding on his fitness, after consulting with medical experts, and the final decision went against the 28-year-old.
"Muriel, Perea and Falcao are not going to be at the World Cup, Jose Pekerman has announced," said a message on the national team's official Twitter account.
Colombia face Greece, Ivory Coast and Japan in Group C at Brazil 2014.