The presenting duo were at the centre of reports in The Sun On Sunday, claiming they’d fallen out while filming the show.
The paper quoted an unnamed source who said they’d clashed over his “over-the-top behaviour” on set, with Zoe said to have “snapped” and shouted for him to “shut up”.
However, the pair made it very clear that there had been no-such incident, cracking jokes about the claims on Twitter.
Posting a link to the story, Rylan wrote: “Morning @ZoeTheBall. You evil witch (this isn’t true btw but made me giggle) x.”
Responding with a picture of them superimposed as feuding Dynasty characters Alexis and Krystle, Zoe wrote: “Love you leggy one xx.”
Rylan the joked: “Can this be our Halloween lewk? X.”
When one fan then sent Rylan a GIF of a fight from kids show Tracy Beaker, he responded: “Where did u get this footage of us this is a breach of privacy.”
Rylan joined It Takes Two last month to help balance Zoe’s work commitments as the host of the BBC Radio 2 Breakfast Show.
Rylan hosts the BBC Two show every Monday and Tuesday, while Zoe presents on Wednesdays and Thursdays, with the pair teaming up to front Friday’s extended episode.
Zoe took over from Claudia Winkleman as the host of It Takes Two in 2011, having previously appeared as a contestant on Strictly in 2005.
Meanwhile, Rylan has also bagged a host of TV jobs in recent months, as he recently fronted a reboot of gameshow Supermarket Sweep and has also been confirmed to present a revival of Ready Steady Cook.
Strictly: It Takes Two airs weeknights at 6.30pm on BBC Two.