Vivienne Jolie-Pitt, five, has previously worked alongside Angelina in the Disney blockbuster Maleficent, and Shiloh, seven, appeared in The Curious Case of Benjamin Button.
But this will be the first time the rest of the Jolie-Pitt clan - Maddox, 12, Pax, 10, Zahara, nine, and Vivienne's twin brother Knox, - will have been on the silver screen.
The film will also be the first time Angelina and Brad have acted together since they kindled their romance on the set of Mr & Ms Smith.
"Their whole family life is rooted in films but Brad and Ange didn't want the children to follow in their footsteps. However, acting is in their genes, and it could turn out to be the start of something big for the next generation," a 'movie insider' told The Daily Mirror.
Angelina, 38, recently talked about her role in Cleopatra and teasingly suggested it might be her last acting project, saying:
"It's one of those that you think maybe that's the one you put everything into and that's where you end it, that's where you finish – in a great way," she said. "What could you do beyond that one?"
The mum-of-six has yet to comment on her children's involvement in the film. But could she be preparing to hand over her acting baton to the next generation of Jolies?
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