Saif al-Islam, the second son of Colonel Gaddafi, has broadcast a statement on the Syrian al-Orouba satellite channel vowing to continue the fight against the National Transitional Council (NTC).
In a vitriolic address, the former regime official, who had been widely tipped to take over from his father, pressed Libyans loyal to Gaddafi to take arms against the NTC and NATO, vowing to “liberate the Green Square”.
"We assure people we are here, ready and in good shape,” he said, claiming to be speaking on a phone from the outskirts of Tripoli.
“We want to tell the people we are present, the resistance is continuing and victory is near.”
He also warned against an attack on Sirte, the final strategic objective for the rebels, saying that 20,000 pro-Gaddafi fighters were ready to defend the town.
"We are going to die in our land," he continued. "We would like to assure people that we are steadfast and the commander is doing well."
"Every Libyan is Muammar Gaddafi, every Libyan is Saif al-Islam. Wherever you find yourself face-to-face with the enemy, fight him."
"We are fine. The leadership is fine and the leader is fine. We are happy, we are drinking coffee and tea with our companions and we are fighting."
In a bizarre twist, Gaddafi’s other son, Saadi, had earlier appeared on the al Arabiya channel claiming that his father had given him the authority to negotiate with the rebels.
Speaking on Sky News, Abdel Hakim Belhadj, the head of the rebel anti-Gaddafi forces in Tripoli, confirmed that he had been in contact with Saadi.
"We were talking about negotiations based on ending bloodshed,” he said.
The mixed messages from members of the stricken regime suggest that Saadi is attempting to negotiate his passage out of Tripoli. Aisha Gaddafi, the Colonel's only biological daughter, fled to Algeria on Monday morning with her mother Safiya and two brothers Mohammed and Hannibal.
For Saif, the possibility of safe transit is far more remote as the International Criminal Court has a warrant out for his arrest.
Evidence has also emerged to suggest that Gaddafi’s adopted daughter, Hanna, believed to have been killed 25-years-ago in an American airstrike, was possibly alive and working as a doctor in Tripoli. The discovery was made following the rebel raid on Gaddafi's compound.
Earlier today, Abdelati Obeidi, Libya's foreign minister,who took over following the defection of Moussa Koussa, was captured on his farm in a suburb of the capital.