Mark Cavendish had urine thrown at him by a spectator during today's time trial in the Tour de France.
Although the Manxman declined to answer questions after completing the 33-kilometres stage from Avranches, his Omega Pharma-Quick Step team confirmed what had happened out on a course packed with fans.
"I regret this. I always felt that cycling fans were gentlemen, enthusiastic people," team manager Patrick Lefevere said. "Mark is sad. He's not upset - just sad. I cannot blame anyone. There are 100,000 or 200,000 people on the road, and one person decided to do this."
The incident came after the British national champion was embroiled in controversy last night when a crash late on stage 10 to Saint-Malo sent Tom Veelers to the floor and ended Cavendish's hopes of a stage win.
Although the Omega Pharma-Quick Step rider was cleared of any blame by the race organisers, many fans condemned his actions on social media.
Cavendish's French team-mate Jerome Pineau was the first to confirm that the reaction had escalated out on the course this morning.
Ashamed when my friend @MarkCavendish said me that he was insulting and pissed all the parcours !!! Shame on you!!
— jerome pineau (@jejeroule44) July 10, 2013
"Ashamed when my friend @MarkCavendish tells me he has been whistled and even sprayed with urine on the course. It's scandalous."
Cavendish remained on the team bus after the stage and offered only a shake of the head when asked to comment on the incident, but another member of staff said with a grin that there had "been a smell" when the Manxman returned to their bus.
Cavendish was irritated by the crash questions on Tuesday
Cavendish was among the early runners in today's time trial due to his lowly position in the general classification, and set a time of exactly 42 minutes.
His team-mate Tony Martin, the world time-trial champion, had the early lead after completing the course in 36 minutes 29 seconds.