John Fashanu has claimed his brother Justin was not gay.
Justin Fashanu, who committed suicide nearly 14 years ago, is the only British footballer to come out as a homosexual.
But his brother contested his sexuality in an on-air interview with talkSPORT.
John Fashanu said: "I don't believe he was gay.
"It's taken [my daughter] a long time to understand that Justin wasn't really gay. He just wanted attention."
His brother came out as gay in October 1990 in an exclusive interview with The Sun newspaper. A week later, his brother John agreed to an exclusive with The Voice, which was headlined: "John Fashanu: My Gay Brother is an outcast."
He revealed he had disowned his brother, however Fashanu expressed regret at the treatment of Justin after his suicide in 1998.
Fashanu's daughter Amal led a BBC documentary called "Britain's Gay Footballers", which aired in January, and was critical of her father's reaction.
"It was very upsetting to see the old news reports of my father disowning his own brother," she says.
"I was only ten when it happened and my parents wanted to protect me. After my parents split up, I grew up with my mother in Madrid and although I heard things, I never discussed it with my father.
"I knew he had not approved of Justin’s lifestyle, but the extent of his disloyalty was a shock," she told The Daily Mail.
Amal has since launched the Justin Campaign, which aims to tackle homophobia in football.