The leader of one of Britain's biggest trade unions has warned Jeremy Corbyn he may be unable to take on the role of prime minister after the new Labour leader said he could never launch a nuclear strike.
Sir Paul Kenny, the general secretary of the GMB, said there was already a "lot of debate" around Mr Corbyn's leadership and whether he could take the party into the 2020 general election.
Mr Corbyn - a long standing campaigner for nuclear disarmament - infuriated senior shadow cabinet colleagues after he said in a BBC radio interview that he would not be prepared to authorise the use of the UK's Trident deterrent if he was prime minister.
Mr Corbyn insisted that even if the party membership voted to retain the current position, he would carry on as leader.
"If I can persuade the whole of the Labour Party to come round to my point of view, I would be very, very happy indeed. I will do my best. If I can't, we'll live with it somehow," he told ITV News.
However Sir Paul, whose union did not endorse any of the candidates in the leadership race, suggested that Mr Corbyn may be unable to take up the post of prime minister if Labour remained committed to multilateral disarmament - with the UK only giving up its arsenal if other nuclear powers agreed to do the same.
"That may well be right," he told Channel 4 News. "At the end of the day, if you are in that role and the party is multilateral and that is the position you are elected to the country, then maybe somebody else should be given the (nuclear) button."
Shadow justice secretary Lord Falconer, who also backs the retention of Trident, said that if Labour decided to keep it, it would only be an effective deterrent if it was accepted by the party leader.
"If the position is that the defensive posture of the UK is to continue to rely on Trident - which I believe that it should be - that is only effective if the head of government accepts that that is the way that it is done, continues to accept the position that there may be circumstances in which it is used," he told BBC2's Newsnight.
The fact that such a senior figure in the Labour movement as Sir Paul is willing to speculate so publicly about whether Mr Corbyn can ever become prime minister is likely to alarm and anger his supporters.
Mr Corbyn was also accused of pre-empting a review by shadow defence secretary Maria Eagle of Labour's current policy - which many Labour MPs and shadow ministers still support - of retaining the deterrent.
Earlier yesterday, on the final day of the Labour Party conference in Brighton, Ms Eagle complained that Mr Corbyn's admission that he would never use nuclear weapons had "undermined to some degree" her review of defence policy.
"I don't think that a potential prime minister answering a question like that, in the way in which he did, is helpful," she said.
Shadow foreign secretary Hilary Benn, who has warned that he would resign if the party adopted a policy of unilateral disarmament, also said that a prime minister had to be to prepared to sanction nuclear action.
"Any prime minister has to have the option because that is the whole theory on which deterrence is based."
Mr Corbyn sought to play down the row, insisting that no decisions were needed until next summer when a Commons vote is expected on Trident renewal.
"We are not in the era of the Cold War any more," he said. "I am opposed to the use of nuclear weapons, I am opposed to the holding of nuclear weapons. I want to see a nuclear-free world. I believe it is possible."
However David Cameron said Mr Corbyn's comments showed Labour could not be trusted with Britain's national security.
"The independent nuclear deterrent that we have in Britain is a vital insurance policy for our nation in what is a very dangerous world," the Prime Minister told reporters during a visit to Jamaica.
"And, frankly, the way the Labour leader has answered that question demonstrates that Labour can't be trusted with our national security which, after all, is the most important duty of government."