Christian bed and breakfast owners Peter and Hazelmary Bull do not just deny double rooms to gay couples.
They also refuse a double bed to unmarried heterosexual couples, they have revealed.
The Bulls say their policy is in accordance with their religious beliefs and was not directed to sexual orientation, but sexual practice.
They were speaking to ITV on the eve of the latest stage of their legal battle against civil partners Martyn Hall and Steven Preddy.
The Bulls had to pay £3,600 in damages to Hall and Preddy, from Bristol, after they were refused a double room at the hotel on religious grounds in September 2008, and have reportedly put the Chymorvah Hotel in Marazion, Cornwall, up for sale following the controversy.
They also said they had been the victims of vandalism and death threats.
The Bulls regard any sex outside marriage as a ''sin'' but deny either direct or indirect discrimination.
They will take their case to the Supreme Court - the highest court in the land - on Wednesday after their original appeal was dismissed by the Court of Appeal.
Speaking to ITV's Daybreak, Mrs Bull said: "We're hoping that in some way or another a pathway will be found through this so that two different lifestyles - which at the moment have had a head-on collision - could live together in our society."
The Bulls say they have been forced to put the nine-bedroom property on the market after a decline in business meant they could not afford the mortgage payments.
Mrs Bull said she found the long-running dispute "thoroughly regretful".
"We're running this under our own roof and our God demands that our faith doesn't end at the kitchen door," she said.
"He means your faith to run in every corner of your life, you can't just section him off like that.
"One would think that dealing with Christians, we would be fair and honest and upright and honourable and live according to the Bible, because the Bible is the Christians' textbook."