Despite being called a "bigot" and "disgusting" by onlookers she continues to abuse them until they leave the train.
She can be heard shouting: "Don't fucking sit on my train and speak about me behind my back in your lingo.
"Do it to my face. I really ain't fucking shy. I don’t know what you’re talking about because you’re doing in behind my back.
"You know English when you want a job. You know English when you want a flat. You know English when you want fucking anything.
"But when you want to try and talk about us on the train you talk in your own lingo."
The apparently unprovoked tirade was recorded on the District Line between Mile End and Plaistow last week.
The video was taken by an onlooker, Jay, 24, who told the Daily Mail: "It was a total shock to witness this racist abuse.
"You really don't expect to see it in this day and age."
By the end of the clip many of the other passengers are laughing at the woman and jeering her.
A British Transport Police (BTP) spokesman said: “We have been made aware of a video posted online which appears to show two men being racially abused onboard a London Underground District line train.
"It is not clear when the video was recorded.
"Officers have launched an investigation and are currently analysing the video.
"Anyone who witnessed the incident, or who has any other information, is asked to contact British Transport Police on 0800 40 50 40, or text 61016, quoting log 147 of 29/03/2015."