The organisation, which advocates a range of rural "sports", such as hunting and shooting, said Packham was pursuing "obsessive crusades", with the BBC pushing "blatant political propaganda".
The Countryside Alliance's chief executive, Tim Bonner, wrote: "There is no issue with people voicing such opinions, but using the position granted by a public service broadcaster to promote an extreme agenda is a different thing entirely."
Bonner continued: "This is the clearest possible abuse of the position the BBC has given Chris Packham and as it is an ongoing behaviour, rather than an isolated incident, it is difficult to see how the situation can change."
Packham, 54, has presented Springwatch since 2009.
In his column in October's edition of BBC Wildlife magazine, Packham criticised conservation groups as being “hamstrung by outdated liaisons with the ‘nasty brigade’ and can’t risk upsetting old friends” in rural communities.
Matt Swaine, editor of BBC Wildlife, said: “Chris Packham is clearly expressing his own views in the column and part of BBC Wildlife magazine's role is to be a forum for exactly this kind of discussion.
“That’s why we will be giving any named charities a right of reply in the November issue and have invited readers to tell us what they think too.”
The reaction on social media has been overwhelmingly supportive of the wildlife presenter and very critical of the Countryside Alliance's attempts to silence him.
Those in support of the Countryside Alliance were much quieter on social media, although some did feel that he should separate his views from his day job.