In her opening monologue, Amy took on female rolemodels, her new film, US gun culture and the Kardashians.
Here's her full eight-minute monologue:
"We have to be a role model for little girls, because who do they have? All they have, literally, is the Kardashians," she said. "Is that a great message for little girls, a whole family of women who take the faces they were born with as a light suggestion?"
The 'Trainwreck' star later starred in a mock PSA video about guns, taking aim at the US's relationship with weapons.