The two were previously thought to be reuniting on an episode of the BBC motoring show - which Matt began presenting in 2016 - later in the month.
However, The Sun has claimed that David has since decided not to appear, following the news that he and his wife are taking a break from their marriage.
An insider said: “Matt tried to get David to come on and it was looking hopeful at one point, despite his busy schedule. But David has now announced he’s on a break from his wife and doesn’t think it’s right to be appearing on a British TV show.
It’s thought David pulling out will be a blow to bosses as, despite a generally positive reception from viewers for the current series, they’re yet to snag an A-list guest for the current run.
According to The Sun, bosses have been left “struggling to find a replacement” for David, with the last two celeb guests of the series, Jamiroquai’s Jay Kay and comedian Ross Noble both having appeared on ‘Top Gear’ in the past.
Matt and David appeared together in ‘Friends’ for 10 years, and after the show ended, David directed two episodes of spin-off show ‘Joey’.
More recently, David made a guest appearance in ‘Episodes’, in which Matt plays a fictionalised version of himself.