Lambesis, whose band has sold over 1m albums, was arrested in San Diego, California, accused of plotting to kill his wife Meggan. The couple have adopted three children from Ethiopia together, but separated last September.
A police spokesman told the BBC: The information came to us late last week. We acted quickly on it. I believe that we averted a great tragedy."
The band are due to tour the US at the end of this month with Killswitch Engage.
As I Lay Dying has stated on numerous occasions that they are all Christians, but not in the traditional sense of being an evangelical or "preachy" outfit. In an FAQ on the band's website, Lambesis says: "If you truly believe something, then it should affect every area of your life. All five of us are Christians."