The 'Mac' image, which also appears on the paper's website, references news that singer Tom Jones will undergo tests in a bid to discover whether he has black ancestry.
It depicts two white colonial-type explorers, one reading a newspaper with the headline "'Am I black?' asks Tom Jones", another approaching two black tribesmen with a test-tube, clutching a briefcase adorned with the words "DNA tests".
Tuesday's cartoon caption reads: "The DNA matches - now just one more test... can you sing Delilah?", a song by Jones that became the sixth best-selling British single in 1968.
Stanley McMurtry 'mac' drew this cartoon, & the Daily Mail published it today. In 2015.November 3, 2015
Twitter users piled in to rebuke the newspaper for publishing Tuesday's picture.
Today's cartoon about Tom Jones' alleged black ancestry shows DailyMail still thinks black people live in the jungle pic.twitter.com/wF6VhJMvpF— joseph harker (@josephharker) November 3, 2015
How does the Daily Mail cover Tom Jones' alleged black ancestry? With a cartoon about black people in a jungle. Vile pic.twitter.com/mc9IDf5kOK— Joseph Willits (@josephwillits) November 3, 2015
Jeez, I just saw that spectacularly racist Mac cartoon from the Mail. Mac? HACK, more like, amirite?— DocHackenbush (@DocHackenbush) November 3, 2015
😒 The Daily Mail's Tom Jones cartoon 😒— Trillson Fisk (@MalachiDennis) November 3, 2015
The Daily Mail has been contacted for comment but did not respond by the time this story went live.