Dave Lee Travis has denied a string of sex charges spanning over 30 years .
The 68-year-old, whose real name is David Patrick Griffin, entered not guilty pleas at London's Southwark Crown Court to a total of 15 charges.
The former Radio One DJ has been accused of assaulting a woman aged over 16 between January 1, 1992 and December 31, 1993, Scotland Yard said.
TOP STORIES TODAY
Wearing a green jacket over a white shirt, he replied "Not guilty" to each of the charges as they were put to him by the clerk.
Travis, of Mentmore in Leighton Buzzard, Bedfordshire, familiar to listeners as DLT, has consistently denied any wrongdoing since he was first arrested on suspicion of sexual offences last November.
He was first charged on August 15 as part of Operation Yewtree, the police investigation prompted by the Jimmy Savile abuse scandal, but the accusations against Travis have no connection to the disgraced television presenter.
He was charged with additional counts on October 1.
Travis is currently on bail on condition that he lives at his Bedfordshire home and does not contact the alleged victims.