Madonna was one of three acts reported to have signed up to headline the festival in 2024.
However, organiser Emily Eavis has urged those who missed out on tickets not to give up hope.
“In these incredibly challenging times, we want to continue to bring you the best show in the world."
Coldplay, Jorja Smith and Haim are among those confirmed to take part.
The festival should have been returning this summer, but had to be axed for the second year running due to the pandemic.
Could the cancellation of the UK's largest festival for the second year running signal the end of the 2021 festival season altogether?