Japanese car giant Nissan would "reconsider" its future investments in Britain if it left the European Union, its chief executive has warned.
Speaking at the launch of Nissan's new Qashqai model, CEO Carlos Ghosn said: ""Obviously it's going to be a major factor happening and we are going to need to consider what does it mean for us for the future.
"If anything has to change we (would) need to reconsider our strategy and our investments for the future."
Nissan's new Qashqai will be built at Nissan's Sunderland site, which employs 6,500 workers. The firm has previously warned against a British exit, with chief operating officer Toshiyuki Shiga saying it would be an "obstacle" for the car giant.
The mounting debate over Britain's membership of the European Union comes as MPs prepare to vote on a bill legislating for a referendum by 2017 if the Tories win the general election in 2015.
John Mills, co-chairman of the Business for Britain group, which is seeking reform of the UK's EU membership, told BBC Radio 4's Today programme: "If Britain were to leave the EU - and that's not what Business for Britain is advocating - I think that Nissan and any other car company would have to take a view as to what the prospects were, particularly if we continue to have free trade with Europe, which is very likely to happen.
"What we are saying is that if there's renegotiation then a much larger proportion of British business would want to stay in. I think Mr Ghosn would be wise to support what we say, which is to push for these negotiations to be successfully accomplished."
Brexiters
Nigel Farage(01 of09)
Open Image ModalThe Ukip leader and MEP is the most famous 'outer'. After his party took over a 100 council seats in May's local election's Nigel Farage is hoping to win the 2014 European elections and then gain MPs in Westminster in 2015. He has confirmed he will seek a parliamentary seat himself. (credit:PA)
Lord Lawson(02 of09)
Open Image ModalMargaret Thatcher's former chancellor and a true 'Tory grandee' revealed in The Times that if and when there is a referendum "I shall be voting out". He also stuck the boot into the David Cameron by saying the prime minister's attempts to renegotiate the terms of the UK's relationship with the EU would be "inconsequential". (credit:PA)
Backbench Brexiters(03 of09)
Open Image ModalThere are quite a few Conservative MPs who would like to wave goodbye to Brussels. Ken Clarke has said the figure is as low as 30 despite the strong eurosceptic feeling on the backbenches. However the exact number is not clear. Mid-Bedforshire MP Nadine Dorries, who remains suspended from the Conservative Party, is currently talk tof the eurosceptic town amid rumours she may defect to Ukip. Other backbench Brexiters include Bill Cash, Douglas Carswell, Peter Bone and Philip Davies and former defence minister Sir Gerald Howarth. (credit:PA)
Labour's 'Let's Leave' Lot(04 of09)
Open Image ModalMost of the anti-EU focus is on the Tory benches. But there are more than a handful of Labour MPs would would like to quit Brussels as well. Eurosceptics include Frank Field, Kate Hoey, Austin Mitchell, and Gisela Stuart.Stuart has argued the status quo is "not sustainable" and Britain should leave. (credit:PA)
Media Moguls(05 of09)
Open Image ModalRupert Murdoch has warned that the EU will "sink" the UK. The News International and boss caused a stir when he met Nigel Farage for dinner in London recently and said the Ukip leader was "reflecting opinion" with his anti-EU views.In November 2010 Richard Desmond’s Daily Express became the first UK newspaper actively to call for Britain to leave the EU, launching a ‘Get Britain Out’ campaign (credit:PA)
I'm A Celeb, Get Us Out Of Here(06 of09)
Open Image ModalOf course no campaign is complete without a bit of star power. The pro-EU camp have Eddie Izzard, who do the Brexiters have?Joan Collins, a 'patron' of Ukip, wants the UK to leave. "The EU, controlled from Brussels, cares only about itself," she said in March. (credit:PA)
The Business Types(07 of09)
Open Image ModalMost business leaders do indeed seem content with what Lawson called the "warm embrace of the European single market", but there are a few dissenters. Private equity guys Jon Moulton and Edmund Truell are two and Next boss and Tory peer Simon Wolfson has said: "Britain should stay in Europe, but only on the right terms". (credit:PA)
The Commentariat(08 of09)
Open Image ModalThere are a number of loud voices whinnying on the sidelines to say "neigh" to the EU notably Melanie Phillips, Richard Littlejohn, Tom Utley, Simon Heffer.Basically the Daily Mail stable. (credit:PA)
Edging Towards Exit(09 of09)
Open Image ModalSeveral high-profile politicians appear to be on the verge of calling for the UK to exit the EU - but just are not there yet.Former defence secretary Liam Fox - pictured here with a big gun - has said "life outside the EU holds no terror" should David Cameron's hopes of negotiating a new treaty fail.Education secretary Michael Gove is said to have told friends the UK has "nothing to be scared of" by leaving Europe.And many other eurosceptic cabinet ministers, including Iain Duncan Smith and Owen Paterson are likely to share that view. (credit:PA)